发明名称 |
METHOD FOR SCREENING PEPTIDES FOR USE IN IMMUNOTHERAPY |
摘要 |
The invention concerns a method for identifying sub-dominant/cryptic epitopes exhibited by a class I HLA molecule, said method comprising at least the following steps: a) selecting, from the sequence of a protein with respect to which it is desired to induce a cytotoxic T lymphocyte response, at least a peptide sequence of 8 to 11 amino acids capable of constituting an epitope of said protein exhibited by a class I HLA molecule, and corresponding to a peptide with low affinity for said class I HLA molecule and non-immunogenic; b) preparing, for each selected sequence, a variant peptide derived from said sequence, by substituting the N-terminal amino acid with a tyrosine residue; c) determining the immunogenicity of each variant peptide obtained at step b) by selecting, among the latter, each immunogenic peptide, generating a CTL response specific to the target cells expressing the protein wherefrom it is derived and identifying the peptide sequence wherefrom said immunogenic peptide is derived. The invention is applicable to screening of peptides for use in immunotherapy. |
申请公布号 |
WO0208716(A2) |
申请公布日期 |
2002.01.31 |
申请号 |
WO2001FR02387 |
申请日期 |
2001.07.20 |
申请人 |
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM);INSTITUT GUSTAVE ROUSSY (IGR);KOSMATOPOULOS, KOSTAS;TOURDOT, SOPHIE;SCARDINO, ANTONIO;GROSS, DAVID, ALEXANDRE |
发明人 |
KOSMATOPOULOS, KOSTAS;TOURDOT, SOPHIE;SCARDINO, ANTONIO;GROSS, DAVID, ALEXANDRE |
分类号 |
A61P31/12;A61P35/00;C07K14/16;C07K14/71;C12N9/12;G01N33/569 |
主分类号 |
A61P31/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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