发明名称 |
METHOD OF PURIFYING CALCIUM ION-BINDING PROTEIN |
摘要 |
A method of purifying a calcium ion-binding protein by the cation exchange method whereby the calcium ion-binding protein is conveniently and efficient ly separated and purified from a liquid containing the calcium ion-binding protein and contaminants without resort to any pretreatment of, for example, adding a chelating agent. More particularly, the method comprises, in the process of purifying a calcium ion-binding protein, adsorbing the protein by a cation exchanger in the presence of calcium ion, washing and then eluting. A calcium ion-binding protein obtained by this method which is substantially free from any impurities.
|
申请公布号 |
CA2385371(A1) |
申请公布日期 |
2002.01.31 |
申请号 |
CA20012385371 |
申请日期 |
2001.07.18 |
申请人 |
JURIDICAL FOUNDATION THE CHEMO-SERO THERAPEUTICAL RESEARCH INSTITUTE;KOWA COMPANY, LTD. |
发明人 |
KOMATSU, KAZUHIRO;KOYANAGI, SATOSHI;SUGAWARA, KEISHIN;YOSHIZAKI, HIDEO;FURUKAWA, SHINICHI;ONCHI, TATSUFUMI;MIZOKAMI, HIROSHI |
分类号 |
C07K14/47;C12N9/64;(IPC1-7):C07K1/18;C07K2/00 |
主分类号 |
C07K14/47 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|