METHOD FOR MEASURING JNK/SAPK ACTIVITY BY PHOSPHORYLATION OF TRANSCRIPTION FACTORS

摘要

The invention relates to a method for measuring the JNK/SAPK activity which is based on the implemation of a kinase reaction with JNK/SAPKs which are obtained from a cell lysate and preferably isolated by immune precipitation in the presence of ATP and GST transcription factors (preferably: GST-c-Jun), and subsequently measuring the amount of phosphorylate GST transcription factors (preferably: p-GST-c-Jun) according to the specific binding of an anti-p-GST transcription factor antibody(preferably: anti-p-GST-c-Jun-antibody).