METHODS FOR PRODUCING 5'-NUCLEIC ACID-PROTEIN CONJUGATES

摘要

Disclosed herein is a method for generating a 5'-nucleic acid-protein conjugate, the method involving: (a) providing a nucleic acid which carries a reactive group at its 5'end; (b) providing a non-derivatized protein; and (c) contacting the nucleic acid and the protein under conditions which allow the reactive group to react with the N-terminus of the protein, thereby forming a 5'-nucleic acid-protein conjugate. Also disclosed herein are 5'-nucleic acid-protein conjugates and methods for their use.