NOVEL NUCLEIC ACID PROBES AND METHOD OF ASSAYING NUCLEIC ACID BY USING THE SAME

摘要

In case where a nucleic acid probe is hybridized with a target nucleic acid, a novel probe (1) in which a single-stranded oligonucleotide is labeled with a fluorescent substance in such a manner that the fluorescent intensity of the hybridization reaction system is decreased after the completion of the hybridization; another novel probe (2) in which a single-stranded oligonucleotide is labeled with a fluorescent substance and a quencher in such a manner that the fluorescent intensity of the hybridization reaction system is increased after the completion of the hybridization and no stem loop structure is formed between the base chain at the site of the labeling with the fluorescent substance and the base chain at the site of the labeling with the quencher; various assay kits and DNA chips with the use of these probes; and a method of assaying a nucleic acid, a method of analyzing polymorphism and a real time quantitative PCR method each by using the same.