发明名称 |
A PCR-BASED MULTIPLEX ASSAY FOR DETERMINING HAPLOTYPE |
摘要 |
The invention provides a method for determining haplotype in a template DNA sequence having a first and a second polymorphic marker comprising combining in a single tube a template DNA sequence, forward primers that are allele-specific for the first polymorphic marker, reverse primers that are allele- specific for a second polymorphic marker, and a Stoffel fragment DNA polymerase; conducting polymerase chain reaction (PCR) amplifications to produce an amplification product; and analyzing the amplification product to identify which pair of said forward and reverse primers generated said amplification product; wherein the haplotype is determined by the identification of the forward and reverse primer pair.
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申请公布号 |
CA2353106(A1) |
申请公布日期 |
2002.01.31 |
申请号 |
CA20012353106 |
申请日期 |
2001.07.27 |
申请人 |
PFIZER PRODUCTS INC., |
发明人 |
SEYMOUR, ALBERT BARNES;AFFOURTIT, JASON PATRICK |
分类号 |
G01N33/53;C12N15/09;C12Q1/68;G01N33/566;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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