Assay for identifying theta2u-globulin-mediated nephropathy

摘要

An immunochemical assay kit and associated methodology for identifying alpha 2u-globulin-mediated nephropathy in rodent toxicity/carcinogenicity studies including the following contents: a primary antibody for binding to alpha 2u-globulin; an enzyme-conjugated secondary antibody for binding to the primary antibody; a chemical substrate for creating a detectable change when contacted with the enzyme-conjugated secondary antibody; a first reservoir for contacting alpha 2u-globulin with the primary antibody; a second reservoir for contacting the primary antibody, the enzyme-conjugated secondary antibody, and the substrate with one another; positive and negative controls samples; and purified alpha 2u-globulin. Also included are methods for preparing buffers and reagents; preparing control samples and unknown samples; performing total protein assays and reagent dilution assays; running a competitive inhibition enzyme-linked immunosorbent assay (ELISA) in control samples and unknown samples; and calculating results.