| 摘要 |
<p>The present invention can be used to detect and characterize a genetic mutations in a nucleic acid sample of interest. The invention involves the use of a detector nucleotide sequence encoding a polypeptide, the activity of which can be attenuated or modified when a target sequence including a mutation is cloned into a linker region of the detector nucleotide sequence. In one aspect, the detector nucleotide sequence encodes a detector polypeptide comprising two or more catalytically-essential domains separated by a linker region, where the catalytically-essential domains can function in concert to catalyze a detectable reaction. A mutation in the linker region that results in the elimination of one of the catalytically-essential domains, either by truncation or by substantially altering its amino acid sequence, will result in a detectable activity loss, thereby signaling the presence of a mutation.</p> |
