| 摘要 |
Provided herein are addressable collections of anti-tag capture agents, such as antibodies, that are used as tools for sorting proteins containg polypeptide tags for which the capture agents are specific . Also provided a re methods of nested sorting using the collections. The methods includes the steps of creating tagged collections of molecules by introducing a set of nucleic acid molecules that encode unique preselected polypeptides to create a library of tagged molecules; either before or after introducing the tags, dividing the library into N divisions; translating each division and reactin g each with one of N capture agent collections, identifying the capture agents bound to the polypetide tags linked to molecules on interest, and thereby identifying the one of the divided collections that contains the molecules o f interest.The method can further include adding a new set of tags and repeati ng the sorting process with the same or a different collection capture agents a nd thereby identifying a protein or molecule of interest.
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