发明名称 |
Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments |
摘要 |
This invention is an integrated instrument for the high-capacity electrophoretic analysis of biopolymer samples. It comprises a specialized high-voltage, electrophoretic module in which the migration lanes are formed between a bottom plate and a plurality of etched grooves in a top plate, the module permitting concurrent separation of 80 or more separate samples. In thermal contact with the bottom plate is a thermal control module incorporating a plurality of Peltier heat transfer devices for the control of temperature and gradients in the electrophoretic medium. Fragments are detected by a transmission imaging spectrograph which simultaneously spatially focuses and spectrally resolves the detection region of all the migration lanes. The spectrograph comprises a transmission dispersion element and a CCD array to detect signals. Signal analysis comprises the steps of noise filtering, comparison in a configuration space with signal prototypes, and selection of the best prototype. Optionally post-processing is done by a Monte-Carlo simulated annealing algorithm to improve results. Optionally, an array of micro-reactors can be integrated into the instrument for the generation of sequencing reaction fragments directly from crude DNA samples.
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申请公布号 |
US2002009741(A1) |
申请公布日期 |
2002.01.24 |
申请号 |
US20010836383 |
申请日期 |
2001.04.17 |
申请人 |
CURAGEN CORPORATION |
发明人 |
SIMPSON JOHN W.;ROTHBERG JONATHAN MARC;WENT GREGORY T. |
分类号 |
B01J19/00;G01N27/447;(IPC1-7):C12Q1/68;C12M1/34;C07K1/26;C02F1/469 |
主分类号 |
B01J19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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