| 摘要 |
In one aspect the present invention provides methods for isolating nucleic acid molecules from a cell, the methods comprising (a) contacting a cell with a solution comprising a biopolymer-degrading enzyme, provided that the biopolymer-degrading enzyme is not a nuclease, and (b) contacting the cell with a solution comprising a hydrophobic surfactant to yield a cell suspension comprising cell, biopolymer-degrading enzyme and hydrophobic surfactant, wherein the hydrophobic surfactant has a critical micelle concentration less than 3.0 mM and the concentration of the hydrophobic surfactant in the cell suspension is at least 0.05% (v/v). In another aspect the present invention provides isolated DNA preparations comprising at least 80% supercoiled DNA. In another aspect the present invention provides isolated nucleic acid preparations having an A260/230 ratio of at least 2.0.
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