METHODS FOR REGENERATING CABBAGE USING HYPOCOTYL AND PRODUCING CABBAGE TRANSFORMED WITH USEFUL FOREIGN GENE

摘要

PURPOSE: A method for regenerating cabbage using hypocotyl and a method for producing cabbage transformed with useful foreign gene are provided to establish a system which is allowed to introduce a foreign gene useful to cabbage. CONSTITUTION: The method for regenerating a cabbage comprises steps of germinating cabbage seed to prepare hypocotyl, isolating the hypocotyl, and regenerating a cabbage using the hypocotyl in the culture media containing silver nitrate(AgNO3). In the method for producing the transformed cabbage, the cabbage seed is firstly disinfected and germinated to prepare hypocotyl. And then, hypocotyl is isolated and pre-cultured. Expression vector comprising a foreign gene(e.g., Agrobacterium) is introduced into the hypocotyl, to allow hypocotyl to transform with the foreign gene. The hypocotyl is used to regenerate a transformed cabbage in the culture media containing silver nitrate, hereby transformed cabbage being produced.