An immunogenic reagent which produces an immune response which isprotectiv e against Bacillus anthracis, said reagent compr ising one or more polypeptide s which together represent up to three domains of the full length Protective Antigen (PA) of B. anthracis or variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant ther eof.The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from E. coli. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.
申请公布号
CA2658217(A1)
申请公布日期
2002.01.17
申请号
CA20012658217
申请日期
2001.07.06
申请人
THE SECRETARY OF STATE FOR DEFENCE
发明人
TOPPING, ANDREW WILLIAM;MILLER, JULIE;WALKER, NICOLA JANE;BAILLIE, LESLIE WILLIAM JAMES;TITBALL, RICHARD WILLIAM;BULLIFENT, HELEN LISA;WILLIAMSON, ETHEL DIANE;HOLDEN, PAULA THOMSON;FLICK-SMITH, HELEN CLAIRE