TRANSPOSON MEDIATED MULTIPLEX SEQUENCING

摘要

The present invention relates to an automated method of transposon-mediated multiplex sequencing of DNA fragments inserted into a vector. It relates mor e particularity to an increased efficiency in such automated methods, where th e increased efficiency is obtained by screening out before the sequencing thos e constructs in which the transposon inserted into the vector sequence. This prevents a waste of time and resources in performing reactions sequencing th e vector instead of the DNA fragments of interest.