IDENTIFICATION OF GENES ESSENTIAL FOR THE SURVIVAL OF <i>HAEMOPHILUS INFLUENZAE</i> THROUGH GENOME SCANNING BY TRANSPOSITION MUTAGENESIS

摘要

Essential bacteria genes and a method for identifying 'essential genes' (i.e., genes which are essential to a bacterium's survival) using an in vitro transposition system, a small (975 bp) insertional element containing an antibiotic resistance cassette and mapping these inserts relative to the deduced open reading frames of H. influenzae by PCR and Southern analysis.