| 摘要 |
The invention relates to a method for the examination of cells (20, 38) in a culture medium, in particular for in-situ microscopy in a bio-reactor, whereby cells in a sample volume (12), the depth (d) of which is defined by windows (14, 16) in the direction of the optical axis of the microscope (18), are microscopically imaged and are automatically recorded and processed, by means of an image processing system (30). Said method is characterised in that the depth (d) of the sample volume (12) is suitable for the size of the cells (20, 38), whereby the separation (d) of the windows (14, 16) is successively reduced and the image size (G) of the cells (20, 38) is simultaneously verified by the image processing system (30), such that a separation value (D) is determined, whereby the image size (G) of the cells (20, 38) corresponds to that where the flattening due to the contact pressure of the windows (14, 16) begins to grow and the separation (d) of the windows (14, 16) is set to said separation (D) for the examination.
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