| 摘要 |
<p>Purified nerve growth factor (NGF) consisting of 116 amino acids from the venom of Naja Kaouthia snake was fragmented by trypsin digestion. The fragment which showed the most activity was named ADESH. Synthetic ADESH was constructed using fifteen amino acids (N L G E H P V C D S T D T W V), ten amino acids (N L G E H P V C D S), and five amino acids (N L G E H) of the fragment from its N-terminal. The synthetic versions mimic the biological activity of the natural NGF. The synthetic ADESH was equally active as the fragment of the native NGF. Antibodies versus ADESH react with natural NGF having 116 amino acids. However, anti-NGF reacts poorly with ADESH. This illustrates that the ten amino acids of ADESH are essentially important for the biological activity of neurite growth. Synthetic ADESH can be immensely useful for treating neurological disorders such as Alzheimer's disease (AD) and Parkinson's disease (PD). ADESH being a small molecule will overcome the hindrance of blood-brain barrier and can be delivered by injection, as well as by nasal insufflation, buccal cavity, etc.</p> |
