| 摘要 |
<p>The invention is directed to methods for purifying Troponin I, particularly recombinant Troponin I produced in a bacterial expression system. Recombinant Troponin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g. by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrathionate yielded sulfitolyzed Troponin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.</p> |
