摘要 |
The present invention provides methods and compositions comprising a population of alphavirus replicon particles comprising two or more isolated nucleic acids selected from 1) an isolated nucleic acid encoding an <i>env</i> gene product or an immunogenic fragment thereof of a human immunodeficiency virus, 2) an isolated nucleic acid encoding a <i>gag</i> gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the <i>gag</i> gene product or immunogenic fragment thereof is modified to inhibit formation of virus-like particles containing the <i>gag</i> gene product or the immunogenic fragment thereof and their release from a cell, and 3) an isolated nucleic acid encoding a <i>pol</i> gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the <i>pol</i> gene product or immunogenic fragment thereof is modified to inhibit integrase, Rnase H and/or reverse transcriptase activity, and wherein the nucleic acids are each contained within a separate alphavirus replicon particle. |
申请人 |
ALPHAVAX, INC.;UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL;OLMSTED, ROBERT;KEITH, PAULA;DRYGA, SERGEY;CALEY, IAN;MAUGHAN, MAUREEN;JOHNSTON, ROBERT;DAVIS, NANCY;SWANSTROM, RONALD |
发明人 |
OLMSTED, ROBERT;KEITH, PAULA;DRYGA, SERGEY;CALEY, IAN;MAUGHAN, MAUREEN;JOHNSTON, ROBERT;DAVIS, NANCY;SWANSTROM, RONALD |