| 摘要 |
<p>Methods, dipsticks and kits for testing for the presence of a target nucleic acid in a sample solution are described. The methods comprise forming a complex between target nucleic acid, a capture probe capable of hybridising to the target nucleic acid, and a detection probe capable of hybridising to the target nucleic acid in the sample solution, then contacting the sample solution with the contact end of a dipstick to cause the complex to travel by capillary action to a capture zone of the dipstick. At the capture zone a capture moiety capable of binding the capture probe by non base pairing interactions captures the complex. Presence of detection probe at the capture zone indicates the presence of target nucleic acid in the sample solution. Thus, only non base pairing interactions occur on the dipstick. Such methods allow rapid and sensitive detection of target nucleic acid.</p> |
