ASSAY METHOD AND SYSTEM FOR IDENTIFICATION OF P2Y RECEPTOR AGONISTS AND ANTAGONISTS

摘要

A method and system for assaying P2Y receptors. The method allows for direct detection of ligand binding events with a high signal to noise ratio. The ability to quantify binding events involving the P2Y receptor has heretofore been unavailable due to unavoidable non-selective binding of a radioligand or other traditional probes. A cell-free system for the study of P2Y receptors including a P2Y receptors; optionally, a protein that is normally associated with the P2Y receptor in nature; and a vesicle, is also disclosed.