发明名称 |
Use of beta-catenin in the expansion of stem and progenitor cells |
摘要 |
Mammalian progenitor or stem cells are expanded in vitro by increasing the levels of beta-catenin in the cell. The expanded cells substantially maintain their original phenotype including the ability to give rise to multiple types of differentiated cells. The intracellular levels of beta-catenin may be manipulated by providing exogenous beta-catenin protein to the cell, or by introduction into the cell of a genetic construct encoding beta-catenin. The beta-catenin may be a wild-type or stabilized mutant form of the protein. Preferably the long term cell culture medium substantially lacks stromal cells and cytokines.
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申请公布号 |
US2002004241(A1) |
申请公布日期 |
2002.01.10 |
申请号 |
US20010766387 |
申请日期 |
2001.01.18 |
申请人 |
REYA TANNISHTHA;NUSSE ROELAND;WEISSMAN IRVING L. |
发明人 |
REYA TANNISHTHA;NUSSE ROELAND;WEISSMAN IRVING L. |
分类号 |
C12N5/02;C12N5/0789;C12P21/04;(IPC1-7):C12N5/08;C12N15/86 |
主分类号 |
C12N5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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