摘要 |
The present invention discloses eight new redueced dimensionality (RD) triple resonance nuclear magnetic resonance (NMR) experiements for measuring chemical shift values of certain nuclei in a protein molecule. The RD 3D <u>HA</u>,<u>CA</u>,(CO),N,HN NMR experiment and the RD 3D <u>H</u>,<u>C</u>,(C-TOCSY-CO),N,HN NMR experiment are designed to yield "sequential" connectivities, while the RD 3D <u>H</u>< alpha beta >,<u>C</u>< alpha beta >,CO,HA NMR experiment and the RD 3D <u>H</u>< alpha beta >,<u>C</u>< alpha beta >,N,HN NMR experiment provide "intraresidue" connectivties. The RD 3D <u>H</u>,<u>C</u>,H-COSY NMR experiment, the RD 3D <u>H</u>,<u>C</u>,C, H-TOCSY NMR experiment, and the RD 2D <u>H</u>,<u>C</u>,H-COSY NMR experiment allow one to obtain assignments for aliphatic and aromatic side chain chemical shifts, while the RD 2D <u>HB</u>,<u>CB</u>;(CG,CD),HD NMR experiment provide information for the aromatic side chain chemical shifts. In addition, a method of conducting suites of RD triple resonance NMR experiments for high-throughput resonance assignment of proteins and identification of the location of secondary structure elements are disclosed. |