摘要 |
<p>The present invention discloses eight new redueced dimensionality (RD) triple resonance nuclear magnetic resonance (NMR) experiements for measuring chemical shift values of certain nuclei in a protein molecule. The RD 3D HA,CA,(CO),N,HN NMR experiment and the RD 3D H,C,(C-TOCSY-CO),N,HN NMR experiment are designed to yield 'sequential' connectivities, while the RD 3D H?αβ,Cαβ¿,CO,HA NMR experiment and the RD 3D H?αβ,Cαβ¿,N,HN NMR experiment provide 'intraresidue' connectivties. The RD 3D H,C,H-COSY NMR experiment, the RD 3D H,C,C, H-TOCSY NMR experiment, and the RD 2D H,C,H-COSY NMR experiment allow one to obtain assignments for aliphatic and aromatic side chain chemical shifts, while the RD 2D HB,CB;(CG,CD),HD NMR experiment provide information for the aromatic side chain chemical shifts. In addition, a method of conducting suites of RD triple resonance NMR experiments for high-throughput resonance assignment of proteins and identification of the location of secondary structure elements are disclosed.</p> |