发明名称 |
HOST CELLS CONTAINING MULTIPLE INTEGRATING VECTORS |
摘要 |
The present invention relates to the production of proteins in host cells, a nd more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors , and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity o f infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening.
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申请公布号 |
CA2413151(A1) |
申请公布日期 |
2002.01.10 |
申请号 |
CA20012413151 |
申请日期 |
2001.06.29 |
申请人 |
GALA DESIGN, INC. |
发明人 |
MILLER, LINDA U.;BLECK, GREGORY T.;YORK, DONNA;BREMEL, ROBERT D. |
分类号 |
C12N15/09;C07K14/02;C07K16/12;C07K16/30;C12N5/10;C12N7/04;C12N15/82;C12N15/85;C12N15/86;C12N15/864;C12N15/867;C12P21/02;C12Q1/02;(IPC1-7):C12N15/85;G01N33/50 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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