ANTISENSE TYPE GENE TRAP VECTOR

摘要

Cells suffering from the deletion or inhibition of the function of expressing a normal gene (wild type gene) in alleles corresponding to a trapped gene which are applicable also to in vitro cell differentiation systems other than ES cells; a method of preparing the same; a gene trap method with the use of the above cells; and an antisense promoter transfer vector to be used in the preparation of the above cells. By transferring the gene trap vector into cells, a trap clone can be efficiently obtained. By inserting a powerful promoter into a definite position in a mutated locus, wherein the insertion of the gene trap vector of the trapped clone has occurred, in the direction opposite to the endogenous gene, moreover, the antisense RNA against the trapped gene is compulsively transcribed and thus the transcription product from the wild type gene is disrupted.