| 摘要 |
A novel cell culture device for the production of metabolites as provided herein. The device includes a housing, and a cell culture space, within the housing. Fresh nutrient medium inlet means and outlet means, are connected, in parallel, to a sheet of tubes forming a wall of the culture apace and which are in fluid communication with the culture space. At least a subset of open-ended permeable capillaries is arranged in a layer within the culture space. The open ends of the capillaries lead to external inlet gas means and to outlet gas means and are in gaseous communication with the interior of the capillaries. The capillaries are arranged at equal intervals so as to form, on one side of the sheet of tubes, one subset allowing a homogeneous distribution for gas exchanges with the cells. A spent medium outlet means is connected to a first micron sieve forming each downstream wall of the cell culture space, in order to ensure that the cells are confined while allowing said spent medium to leave. The sheet of tubes in connection with the fresh nutrient medium inlet means and the outlet means, are structured in a closed-loop circuit. The sheet of tubes is constructed so as to provide, when fresh nutrient medium is passing through the circuit, simultaneously a first flow of fresh medium through eachof the tubes, from end to end, and a second flow of fresh medium which is perpendicular to the first flow, from the tubes to the cell culture space, through their permeable walls.
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