Hybrid genes incorporating a DNA fragment containing at least one gene encoding an insecticidal protein and a gene encoding a glutamine synthase inhibitor, plasmids, transformed cyanobacteria expressing such proteins and method for use as biocontrol agent

摘要

Cyanobacteria incorporating a gene from Bacillus sp. encoding for insecticidal proteins (endotoxins) is described. The endotoxins are particularly effective against Diptera (mosquito) larvae. Recombinant vectors for transforming DNA fragments of the endotoxin gene or genes into the Cyanobacterium are described. The Cyanobacteria are easily grown in ponds or the like where the mosquitos or other insects breed. Additionally, these Cyanobacteria contain and express beside the endotoxin gene(s), a determined gene, the expression of which inhibits the herbicidal effects of glutamine synthetase inhibitors, enabling the Cyanobacteria to be selectively protected from competitive Cyanobacteria by treating them with the glutamine synthetase inhibitors