发明名称 |
MEASURING VIRAL REVERSE TRANSCRIPTASE ACTIVITY |
摘要 |
In clinical settings as well as in a drug-discovery context, the impact of an agent that may affect reverse transcriptase (RT) can be measured and even quantified by bringing a sample, which may contain an RT, into contact with an RNA template, a primer complementary to the RNA template, and appropriate oligonucleotide-specific primers, under conditions such that they react, in the presence of RT, to form a cDNA product in inverse proportion to the effect of the agent. The amount of any resultant cDNA product then can be measured. The approach is readily implemented as a real-time, quantitative kinetic assay for RT activity. |
申请公布号 |
WO0198541(A2) |
申请公布日期 |
2001.12.27 |
申请号 |
WO2001US19763 |
申请日期 |
2001.06.21 |
申请人 |
HARRIS, ROBERT, B.;REYNOLDS, THOMAS, R. |
发明人 |
HARRIS, ROBERT, B.;REYNOLDS, THOMAS, R. |
分类号 |
C12Q1/48;C12Q1/68;C12Q1/70 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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