| 摘要 |
Disclosed is a method for detecting 5-methylcytosine in genomic DNA samples. First, genomic DNA from a DNA sample is chemically reacted with a reagent, 5 - methylcytosine and cytosine reacting differently. The pre-treated DNA is the n ampified with primers from a different sequence using a polymerase. In the following step, the amplified genomic DNA is hybridised to an oligonucleotid e array and PCR products are obtained which must be provided with an identifyi ng mark. Alternatively, the PCR products can be extended in a Primer Extension Reaction, the extension products also being provided with an identifying mar k. The last step involves examining the extended oligonucleotides for the presence of the identifying mark.
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