DETECTION OF BIOMOLECULES IN GELS FOLLOWING ELECTROPHORESIS

摘要

The present invention comprises method of detecting biomolecules in situ in gel separation medium following electrophoresis that provides sensitivity similar to blotting. This method is applicable to in situ detection of a wide range of biomolecules including proteins and nucleic acids. After electrophoretic separation of a sample in gel separation media comprising a gellable polymeric material other than cross-linked polyacrylamide, the gel is contacted with a solution comprising at least one detectably labeled reagent directed to a biomolecule under conditions suitable for binding of the reagent to the biomolecule. Alternatively, the gel is contacted with a solution comprising at least one non-detectably labeled reagent directed to a biomolecule and a solution comprising at least on detectably labeled reagent directed to a biomolecule. In either case, binding of the detectably labeled reagent is assessed and indicates detection of biomolecules in the gel.