METHODS AND MEANS FOR THE COMPLEMENTATION OF VIRAL PROTEIN EXPRESSION IN STABLE CELL LINES

摘要

Method for the propagation of recombinant virus in packaging cells, comprising the steps of: culturing the packaging cells free of virus introduction of the virus in the packaging cells propagation of the virus within the packaging cells isolating the virus from the packaging cells, wherein at least one gene of the virus, the expression of which leads to a product, being essential for the replication of the virus within the packaging cells is modified to be not functional or at least partly deleted from the said virus, the packaging cells comprise a stably replicating complementing gene, coding for a function complementing for the said essential product, the product being toxic to the said packaging cells, the expression of the said complementing gene being under the control of a transactivator responsive element (TRE), the gene encoding the corresponding transactivator being present in the recombinant virus genome and being expressible in the packaging cells upon infection thereof by the said virus.