METHOD FOR DETECTING DIFFERENCES IN GENE EXPRESSION OF WILD TYPE PLANTS AND GENETICALLY MODIFIED PLANTS

摘要

The present invention relates to a method for detecting differences in gene expression between wild type plants and newly developed plants, comprising of: a) providing at least one micro-array which is provided with a number of spots of target material; b) proividing at least one probe which comprises test material of a wild type plant, and a probe which comprises test material of a newly developed plant; c) placing on the micro-array the target material spotted thereon in contact with the probes; d) determining per spot whether hybridization of the probe of the wild type plant with the target material has taken place and determining per spot whether hybridization of the probe of the newly developed plant with the target material has taken place, in order to determine whether there is a difference in gene expression between the wild type plant and the newly developed plant, characterized in that the test material comes from plants which have been subjected to a treatment prior to isolation of the test material. With the method according to the invention it is possible to demonstrate in simple manner to what extent the gene expression of a newly developed plant differs, as a consequence of a worst case treatment, from the corresponding wild type plant.