| 摘要 |
In carrier-free continuous deflection electrophoresis problems are encountered in the collection of fractions and it is necessary to use hose pumps with a number of channels corresponding to the number of fractions desired. This arrangement is not only elaborate and susceptible to disturbance, for a great variety of reasons, but even more importantly it necessitates a certain minimal flow velocity within the separation chamber. To improve the effectiveness of separation it is proposed by the present invention to adjust the outflow rate from the separation chamber in the region of its outlet end by introducing an additive medium with a predetermined flow rate in countercurrent to the flow direction of the separation and sample media. In this way the time the sample spends in the electric field and hence the corresponding separation effect is considerably increased. |
