发明名称 |
Methods and reagents for identifying inhibitors of proteolysis of membrane-associated proteins |
摘要 |
The present disclosure provides DNA constructs, genetically modified host cells and methods for identifying inhibitors of proteolysis of membrane-associated protease substrates including, but not limited to, amyloid precursor protein, a Notch protein and sterol response element binding proteins. The methods rely on chimeric proteolytic substrates containing a portion of the proteolytic substrate protein (including the proteolytic cleavage site) and a transcription repressor protein portion. Release of the repressor results in blockage of the transcription of a chimeric reporter gene which includes repressor-responsive transcription regulatory sequences and a reporter protein coding region. A specific protease inhibitor is identified by increased reporter protein activity.
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申请公布号 |
US6333167(B1) |
申请公布日期 |
2001.12.25 |
申请号 |
US20000522666 |
申请日期 |
2000.03.10 |
申请人 |
AMERICAN HOME PRODUCTS CORP. |
发明人 |
QUINET ELAINE M.;SHUEY DAVID J. |
分类号 |
C12N15/62;C12N15/63;C12Q1/37;C12Q1/68;G01N33/50;(IPC1-7):C12Q1/37 |
主分类号 |
C12N15/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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