发明名称 |
MUTANT NIA PROTEINASE WITH REDUCED SELF-CLEAVAGE ACTIVITY AND METHOD OF PURIFICATION |
摘要 |
<p>The present invention provides a mutant 27 kDa NIa proteinase having reduced self-cleavage activity relative to the self-cleavage activity of its wild-ty pe proteinase. The mutant has the same substrate cleavage activity as the wild- type proteinase but is more stable than the wild-type proteinase. The presen t invention also provides a method of obtaining large quantities of active 27 kDa NIa proteinase for use as a tool for purification of other proteins.</SD OAB></p> |
申请公布号 |
CA2435381(A1) |
申请公布日期 |
2001.12.20 |
申请号 |
CA20012435381 |
申请日期 |
2001.06.11 |
申请人 |
YALE UNIVERSITY |
发明人 |
DOUDNA, JENNIFER A.;LUCAST, LOUISE J.;BATEY, ROBERT T. |
分类号 |
C07H21/04;C12N9/50;(IPC1-7):C12N9/50;C07K1/36;C12P21/02;C12N15/62 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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