IN VIVO ASSAY FOR TESTING THE PHENOTYPIC STABILITY
摘要
<p>An <i>in vivo</i> assay to measure anchorage-independent growth and phenotypic stability of a certain cell population comprising subcutaneous or intramuscular injection in a mammal of a cell suspension of articular chondrocytes in an iso-osmotic liquid, the same suspension comprising articular chondrocytes in an amount equivalent to at least 1 x 10<6> chondrocytes as applied to immune-deficient mice. The outcome is linked to molecular markers. The present invention further relates to DNA chips and diagnostic tools comprising the latter to predict the outcome of ACT. Antibodies raised against positive and negative markers of chondrocyte stability can also be used for quality control on the chondrocytes. Therapeutical composition comprising stable chondrocytes are very useful for tissue repair.</p>
申请公布号
WO0124833(A3)
申请公布日期
2001.12.20
申请号
WO2000BE00118
申请日期
2000.10.06
申请人
TIGENIX N.V.;LUYTEN, FRANK;DE BARI, COSIMO;DELL'ACCIO, FRANCESCO
发明人
LUYTEN, FRANK;DE BARI, COSIMO;DELL'ACCIO, FRANCESCO