METHOD OF CONSTRUCTING scFv ANTIBODY FUSED WITH FLUORESCENT PROTEIN

摘要

A method of constructing an scFv antibody having a fluorescent protein fused therewith and showing an activity of binding to an antigen. An scFv antibody library composed of phage clones with the expression of scFv antibody on the surface is prepared. From this library, a clone expressing an antibody recognizing a specific antigen is screened. Then, an scFv gene is obtained from this clone and transferred into an expression vector in which a gene encoding a fluorescent protein has been preliminarily integrated. By expressing the vector in a host, an scFv antibody having the fluorescent protein fused therewith can be produced.