发明名称 |
METHOD FOR QUANTITATING CHOLESTEROL |
摘要 |
<p>A method of selectively quantitating cholesterols, comprising determining the amount of cholesterols in a measuring lipoprotein in a sample in the presence of a compound having a relatively strong affinity with non-measuring lipoproteins in the sample, a surfactant exhibiting a relatively strong action on the measuring lipoproteins, and a cholesterol determination reagent; a method of selectively determining the amount of cholesterols comprising preferentially reacting the cholesterols present in non-measuring lipoproteins in a sample in the presence of a compound having a relatively strong affinity with the measuring lipoprotein in the sample, a surfactant exhibiting a relatively strong action on the non-measuring lipoproteins, and a cholesterol determination reagent, and determining the amount of cholesterols in the remaining measuring lipoprotein; and a reagent for quantitative determination of cholesterols comprising, separately or as a mixture, a compound having a relatively strong affinity with one of the lipoproteins in the sample for carrying out the above methods, a surfactant exhibiting a relatively strong action on the other lipoproteins, and a cholesterol determination reagent are disclosed. The methods and reagents ensure efficient quantitative determination of cholesterols in specific lipoprotein fractions by a simple procedure without requiring a pretreatment such as centrifugation. The methods and reagent can be applied to various types of automated analyzers.</p> |
申请公布号 |
EP1164376(A1) |
申请公布日期 |
2001.12.19 |
申请号 |
EP20000909725 |
申请日期 |
2000.03.17 |
申请人 |
DAIICHI PURE CHEMICALS CO. LTD. |
发明人 |
YAMAMOTO, MITSUAKI;TAKAHASHI, YOKO;TANIGUCHI, YURIKO;ODAHARA, SHOKO;NAKANISHI, KAZUO;NAKAMURA, MITSUHIRO;HINO, KOICHI |
分类号 |
G01N33/92;(IPC1-7):G01N33/92 |
主分类号 |
G01N33/92 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|