Reverse detection for identification and/or quantification of nucleotide target sequences on biochips

摘要

The present invention relates to a method for the identification and/or the quantification of one or more target nucleotide sequences (3) present in a sample and discrimination from possibly homologous sequences, by using two sets of nucleotide sequences (1 and 2), characterised in that the first set of nucleotide sequences possibly labelled (1) bind specifically to said target nucleotide sequences (3) in a first step and are detected and/or quantified through hybridisation with the second of capture nucleotide sequences (2) having at least a part of sequence complementary to the possibly labelled nucleotide sequence (1) of the first set of nucleotide sequences, said capture nucleotide sequence (2) being immobilised upon the surface (4) of a solid support according to an array of at least 4 discrete regions/cm<2>, each of said discrete regions being bonded with one species of capture nucleotide sequences (2), and the identification and quantification of the binding between the possibly labelled nucleotide sequence (1) and their corresponding capture nucleotide sequence (2) is correlated with the identification and the quantification of a target nucleotide sequence (3) present in the sample. <IMAGE>