| 摘要 |
The invention provides a method for obtaining a patch clamp recording from a cell, which includes the steps of providing a microchannel capable of an axi al flow of a liquid, providing in the microchannel at least one access port to allow radial access from the exterior of the microchannel (air) to the interior of the microchannel (liquid); whereby liquid in the microchannel forms a meniscus at the port and produces an air/liquid interface, providing a patch-clamp pipette having a tip suitable for passing into the access port suitable for forming a high-resistance (giga-ohm) electrical seal between th e tip and the cell, passing liquid carrying the cell axially along the microchannel, causing the cell to be carried to the access port, moving the patch-clamp pipette tip and the microchannel relative to each other radially to bring the tip into contact with the air/liquid interface in the access port, applying suction to the patch-clamp to draw the cell onto the tip to form the seal, and making a patch-clamp recording. The invention also provid es an apparatus for carrying out the method.
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