| 摘要 |
A method for analysing spots in a gel includes the step of digitally sieving the spots to group them according to their radius. Before digitally sieving the spots a computer means digitises an image of the gel and identifies the spots. A segmentation algorithm is used to digitise the image. The spots are grouped from the smallest radius R1 which is equivalent to a spot having a diameter of a single pixel up to Rn where Rn is the diameter of the largest spot in the image with each group being one pixel larger than the last. This process allows a user to analyse the image of the gel according to spot size. One particularly useful application is to remove all the very small radius R1 spots from the image as these tend to be unwanted protein fragments and by removing these spots or noise from the image improves the quality of the image. This removal of background "noise" improves the accuracy of the excision apparatus to allow it to excise relatively pure samples. |
