| 摘要 |
The device of present invention is used for cutting and recovery of a selected gel piece from a larger gel mass. The gel piece suitably contains molecules that will be used in further work. The preferred device is made up of at least two parts: a hollow member with a distal end terminating in a cutting edge, a proximal end, and a lumen in between the proximal and distal ends; and a piston member. The lumen part of the hollow member close to the proximal end has a larger cross section than a lumen part close to the distal end. The piston body has at least a first body that fits snugly in the lumen part having the larger cross section and a second body is longer than length of the lumen part with a smaller cross section and fits within the smaller cross section portion of the hollow tube, creates reduced pressure when moved towards proximal end of the hollow tube is disposed within the lumen. After cutting a selection portion of the gel mass with the cutting end of the hollow member, and thereby forcing a cut portion of the gel mass into the smaller cross section portion of the lumen, movement of the piston away from the cutting edge reduces the pressure in the smaller cross section of the lumen and this serves to keep the cut gel piece in lumen of the hollow member. The cut gel can then be ejected by reversing the movement of the piston. In a preferred embodiment, the cut gel portion can be recovered. |
