发明名称 |
Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of Leptin. |
摘要 |
A method for detection of a compound that mimics, potentiates or inhibits the physiological effect of leptin, which method comprises: (a) for a compound which mimics the physiological effect of the leptin, assessing the effect of the compound upon a leptin activated signal transducer and activator of transcription (STAT) DNA response element coupled to a reporter gene; or (b) for a compound which potentiates or inhibits the physiological effect of leptin, assessing the effect of the compound upon the response provided by leptin upon a leptin activated STAT DNA response element coupled to a reporter gene; wherein, the response element and the reporter are expressed in the human Caucasian colon epithelial cell line (CACO-2) or the response element and the reporter are expressed in CACO-2 cells, which cell line (the engineered cell line) is also transfected with a polypeptide which is capable of mediating or enhancing the stimulation by leptin of a leptin activated STAT DNA response element and contains the appropriate STAT proteins. |
申请公布号 |
ZA200007689(B) |
申请公布日期 |
2001.12.12 |
申请号 |
ZA20000007689 |
申请日期 |
2000.12.20 |
申请人 |
THE UNIVERSITY OF BUCKINGHAM |
发明人 |
CAWTHORNE, MICHAEL, ANTHONY;VALUR EMILSSON;MORTON, NICHOLAS, MICHAEL |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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