METHODE DE PRODUCTION ET DE CRYOCONSERVATION D'EMBRYONS BOVINS DEVELOPPES IN VITRO ET PAILLETTE CONGELEE POUR LE TRANSFERT DIRECT DE CEUX-CI

摘要

The invention concerns a method for producing cryopreserved bovine embryos from bred oocytes in vitro comprising the following steps: 1) maturing and breeding bovine oocytes in vitro; 2) culturing in vitro said bred oocytes until the morula or blastocyst stage; 3) selecting quality 1 embryos; 4) freezing the selected embryos. The culturing step (2) is a co-culture on a mat of Vero cells carried out serum-free, and the freezing step (4) is carried out while the embryo is conditioned in a freezing medium containing a glycerol-trehalose mixture as cryoprotectant.