Verfahren für die Bestimmung von Substrat- und Produktkonzentrationen in einem Medium

摘要

The invention relates to a method for the determination of product- and substrate-concentrations in liquid and/or gaseous media, whereby several probes withdraw at least one substance for analysis - the analyte from at least one sampling region (3), across a semi-permeable membrane (2), by time-controlled diffusion of the at least one analyte between the relevant medium and a diffusion medium, which is fed to the sampling regions (3) through fluid lines (5a, 5b), by means of at least one pump (6). The diffusion medium is removed from the sampling region, with concomitant introduction of new diffusion medium and led to at least one detector (7) and analysed by the same to determine analyte concentration. The invention is characterised in that the detector provides a time-dependent concentration distribution, or a time-dependent distribution of a signal proportional to the concentration, which determines a change in the ratio of the signal maximum to the baseline in the output of the detector signal, thus revealing any change in the diffusion properties of the semi-permeable membrane and determining a correction factor.