USE OF CODON-VARIED OLIGONUCLEOTIDE SYNTHESIS FOR SYNTHETIC SEQUENCE RECOMBINATION
摘要
Methods of providing recombination libraries that include codon-varied oligonucleotide sequences are described. Codon-varied oligonucleotides are synthesized using trinucleotide or mononucleotide phosphoramidite sequences, and are derived from homologous or non-homologous nucleic acid sequences, or combinations of such sequences. Various methods of recombining codon-varied oligonucleotide sequences to expedite artificial evolution are also described. The present invention additionally relates to various integrated systems that are optionally used to automate these recombination methods.
申请公布号
WO0123401(A3)
申请公布日期
2001.12.06
申请号
WO2000US26708
申请日期
2000.09.28
申请人
MAXYGEN, INC.;WELCH, MARK;NESS, JON, E.;GUSTAFSSON, CLAES;STEMMER, WILLEM, P., C.;MINSHULL, JEREMY
发明人
WELCH, MARK;NESS, JON, E.;GUSTAFSSON, CLAES;STEMMER, WILLEM, P., C.;MINSHULL, JEREMY