NUCLEOTIDE ANALOGUES COMPRISING A REPORTER MOIETY AND A POLYMERASE ENZYME BLOCKING MOIETY

摘要

Nucleotides comprising a reporter moiety and a polymerase enzyme blocking moiety in which the reporter moiety does not also act as a polymerase enzyme blocking moiety are described. Also described are compounds of Formula (I): wherein W is a phosphate group, B is a base, Y is a linker comprising an enzyme-cleavable group, R<2> is a reporter moiety, R<3> is selected from H or OH, Z and Z' are selected from H, OH, or a group X-R<1>, wherein X is a linker comprising an enzyme-cleavable group and R<1> is a polymerase enzyme blocking group, provided that at least one of Z and Z' is X- R<1>.