METHOD FOR CONVEYING BNYVV RESISTANCE TO SUGAR BEET PLANTS

摘要

The present invention relates to a method for conveying resistance to beet necrotic yellow vein virus (BNYVV) to a sugar beet plant, which method comprises the following steps: (a) preparing a DNA fragment of at least 15 nucleotides in a sequence that is essentially homologous to the corresponding nucleotide sequence of the genomic RNA 1 of the beet necrotic yellow vein virus (BNYVV), (b) introducing said DNA fragment, operately linked to a promotor that is active in sugar beet plants, into a sugar beet plant cell to obtain a transformed sugar beet cell; and (c) regenerating a transgenic sugar beet plant from the transformed sugar beet plant cell. In addition, the invention relates to a DNA vector harboring a fragment of at least 15 nucleotides in a sequence that is essentially homologous to the corresponding nucleotide sequence of the genomic RNA 1 of BNYVV and transcription and translation regulatory sequences operably linked therewith, as well as to the use of said DNA vector for the transformation of a plant cell. The invention further relates to a plant cell comprising in its genome a DNA fragment of at least 15 nucleotides in a sequence that is essentially homologous to the corresponding nucleotide sequence of the genomic RNA 1 of BNYVV and the use of said plant cell for the regeneration therefrom of a sugar beet plant that is resistant against BNYVV.