| 摘要 |
1. Method of carrying out cell transfection and achieving bio-accessibility of genetic material in vitro and ex vivo - Characterized in that: The cells to be transfected are contacted with a dendritic polymer complex containing genetic material, where the ratio of the genetic material to the dendrimer is within the range between about 1:1 and about 1:100, while the process is carried out in the solution of DEAE-dextran or glycerine. The dendritic polymer constitutes at least one solvent-soluble radially-symmetrical dendrimer, which contains at least one branch leaving from the core and containing at least one final group, while (1) the ratio of final groups to core branches is equal to two or more; (2) the density of final groups per polymer volume unit is at least 1.5 times larger than the density of a conventional elongated star-shaped polymer with a similar core and monomer components, with a comparable molecular weight and number of branches, while each branch of the elongated conventional star-shaped polymer contains only one final group; and (3) the molecule volume, determined on the basis of dimension examination by means of scalable molecule models of Corey-Pauling, constitutes not more than about 80 percent of the volume of the elongated conventional star-shaped polymer, and the polymer contains regular dendritic branches. 3. The method of carrying out cell transfection and achieving bio-accessibility of genetic material in vitro and ex vivo - Characterized in that: A complex of genetic material with the first dendritic polymer is produced. The dendritic polymer constitutes at least one solvent-soluble radially-symmetrical dendrimer, which contains at least one branch leaving from the core and containing at least one final group, while (1) the ratio of final groups to core branches is equal to two or more; (2) the density of final groups per polymer volume unit is at least 1.5 times larger than the density of a conventional elongated star-shaped polymer with a similar core and monomer components, with a comparable molecular weight and number of branches, while each branch of the elongated conventional star-shaped polymer contains only one final group; and (3) the molecule volume, determined on the basis of dimension examination by means of scalable molecule models of Corey-Pauling, constitutes not more than about 80 percent of the volume of the elongated conventional star-shaped polymer, and the polymer contains regular dendritic branches. Then, the complex is placed in a solution containing the second dendritic polymer, as defined above, but with larger molecules than in the case of the first dendritic polymer. The obtained complex and the second dendritic polymer are contacted with the cells to be transfected.
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